Generation of transplantable, functional satellite-like cells from mouse embryonic stem cells.

Functional Concentrations of BMP4 on Differentiation of Mouse Embryonic Stem Cells to Primordial Germ Cells

Evaluation and Comparison of the Expression Levels of the ZBTB16 (Plzf) and ZFP Genes and Alkaline Phosphatase in Three Cell Populations: Mouse Spermatogonial Stem Cells, Embryonic Stem-Like Cells (Es-Like), And Embryonic Stem Cells

Introduction: One of the vital enzymes during spermatogenesis, which is one of the pluripotency factors of stem cells and contributes to maintaining their pluripotency is alkaline phosphatase. ZBTB16 and ZFP proteins are critical elements in stem cells which are expressed in pluripotent stem cells and maintain their pluripotency due to their role in messaging pathways. Material & Methods: The ...

CFU-GM Like Colonies Derived from Embryonic Stem Cells Cultured on the Bone Marrow Stromal Cells

The aim of this study was to isolate mouse embryonic stem cells from late blastocyst stage embryos and to use them as a model system for the study of hematopoietic induction outside the embryo by coculturing of embryonic stem cells with bone marrow stromal cells. Blastocyst stage embryos from pregnant NMRI mice were obtained and cultured for 1-2 days in DMEM medium. The inner cell masses formed...

Establishment, Culture and Freezing of Human and Mouse Embryonic Stem Cells: a Protocol Guide

Studies of the biology of human embryonic stem cells (hES cells) have developed rapidly over the past nine years since the first reports of their derivation. They clearly offer enormous potential, not only for regenerative medicine, but also for drug discovery and toxicology, human developmental biology and cancer research. Realizing these potentials a better understanding of the fundamental as...

The effect of Fibroblast Growth Factor-2(FGF-2) and retinoic acid on differentiation of mouse embryonic stem cells into neural cells

Introduction: Embryonic Stem (ES) cells as pluripotent cells derived from the inner cell mass of blastula can differentiate to neural cells in vitro and this property is valuable in studies of neurogenesis and in the generation of donor cells for transplantation. In this regard, the propose of this research, was the study of the role of two important factors in the development of neural syst...